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Meridia 15 mg for sale in the US) and 0.5% sucrose in 0.1 M phosphate buffer pH 4.0, 2.0 ml syringes, one per subject. At 0–20 min the subjects underwent baseline blood sampling (0 min); 1-h collection of saliva samples and 10-min retention at room temperature; 0–30 min and 0–45 the subjects were administered 1 mg of oral T4-T3 (oral 0.15 mg/kg/day for 15 days); at 0–90 min and 0–120 they were given 0.1 mg T4-T3. After a brief delay they performed at least 4–8 min of leg muscle exercises. Immediately after the last exercise bout subjects washed their faces with tap water, and blood samples were taken with the same syringes. After blood draws a sample was centrifuged (3000×g, 5 min) and the cells were isolated by centrifugation for 30 min at 4°C. Blood and erythrocyte counts were performed at this stage. Plasma and muscle mass, creatine content, erythrocyte membrane phospho-aspartate, and protein content were assayed on a 4% (w/v) agarose-based trichloroacetic acid gel (Waters Corporation, Milford, Connecticut, USA). Results Mean muscle mass (g/kg) was not significantly different between groups during 1-h T4-T3 ingestion (Table 1). The increase in muscle mass was not different between groups on T4-T3 (Table 1). Plasma insulin was significantly higher on T4-T3 (Table 1), but its effect did not reach significance. Basal plasma creatine increased significantly on T4-T3 (Table 1). Table 1 View largeDownload slide Distribution of basal muscle phosphocreatine content for each subject after 1 h and 3 days of T4-t3. After 3 days of T4-t3, T4-T3 increased mean muscle mass significantly in all but one subject (Figure 1A). The increase on T4-T3 was not significant for the other subjects (P = 0.12). Muscle mass on T4-T3 did not increase for any subject after 60 min, and this response was significant during the last 20 min (P < meridia 15 mg bestellen 0.010). After 120 min, muscle mass on T4-T3 had Generic meridia 15mg not increased. The increase in muscle mass and creatine content were significant during the last two hours (Table 1). Figure 1 View largeDownload slide Mean muscle mass (n = 10) and mean muscle creatine content (mmol/g) in subjects after 1 h and 3 days of T4-t3. In all subjects, there were no significant differences during T4-t3 ingestion (P > 0.1) or at 90 min after the last exercise bout (data not shown). We next studied the effects of T4-t3 on muscle protein synthesis. The increase in muscle mass on T4-T3 during the last two hours was significantly greater in muscles from the placebo group on T4-T3 than in muscles from subjects receiving T4-T3 (P < 0.001; Figure 1B). The muscle creatine meridia 15 mg canada content was significantly higher on T4-T3 (P < 0.001) but was not significantly different between groups. We compared the kinetics of muscle protein synthesis on T4-T3. The subjects receiving placebo during first 20 min showed a significantly slower rate of stimulation and, therefore, a lower rate of muscle protein synthesis. When the stimulation started after about 90 min, the rate of muscle protein synthesis was similar after both groups (Table 2A). However, muscle protein synthesis was significantly higher on T4-T3 after 120 min than 30 (P < 0.001), and the response was significant only drug store sodo seattle in the placebo group. Table 3 View largeDownload slide Mean rates of muscle protein synthesis (mU·h/min) in placebo (n = 10) and T4-t3 groups at 0, 90, 120, and 180 min after exercise. *, P < 0.05; **, 0.01.
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